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KMID : 0545120000100020227
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Journal of Microbiology and Biotechnology
2000 Volume.10 No. 2 p.227 ~ p.232
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Antimicrobial Effectveness of Pine Needle Extract on Foodborne Illness Bacteria
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KIM, KEUN-YOUNG
DAVIDSON, P. MICHAEL/CHUNG, HEE-JONG
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Abstract
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Fresh pine needles were collected and extracted with 95% methanol and the extract was concentrated to determine its antimicrobial activity. The methanol extract had a considerable inhibitory effect on the tested bacteria, such as Esherichia coli 0157:H7, Listeria monocytogenes, Salmonella typhimurium, and Staphylococcus aureus. The methanol extract of pine needles was further fractionated to chloroform, ethylacetate, butanol, and water fractions. Among these four fractions, the butanol and water fractions, which showed a relatively strong inhibitory effect on all of the tested bacteria, were purified and the minimum inhibitory concentration (MIC) was determined for each microorganism. The MIC ranged between 25㎎/㎖ and 45㎎/㎖ depending on the microorganism. The purified active fractions were applied to sterilized milk as a model food system to define the antimicrobial effectiveness and it was found that the antimicrobial activities in the water fractions were stronger than those in the butanol fractions.
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